micro bio spin p6 chromatography column (Bio-Rad)
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micro bio spin p6 chromatography column
Micro Bio Spin P6 Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p6+micro+bio+spin+chromatography+column/Micro+Bio-Spin+P-6+Gel+Columns/pm41315772-385-0-6
Average 96 stars, based on 644 article reviews
Micro Bio Spin P6 Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p6+micro+bio+spin+chromatography+column/Micro+Bio-Spin+P-6+Gel+Columns/pm41315772-385-0-6
Average 96 stars, based on 644 article reviews
micro bio spin p6 chromatography column - by Bioz Stars,
2026-10
96/100 stars
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Incubation:Article Title: SNM1A ( DCLRE1A ) is required for the efficient repair of complex DNA breaks in human cells Article Snippet: .. Substrates were prepared as follows: 10 pmol of DNA oligonucleotide (Eurofins MWG Operon, Germany) were 3’-end labelled with 3.3 pmol of α-32P-dATP (Perkin Elmer) using terminal deoxynucleotidyl transferase (TdT, 20 U; ThermoFisher Scientific), incubated together at 37°C for 1 h. To remove unincorporated nucleotides, these were passed through a Article Title: A phosphate binding pocket is a key determinant of exo- versus endo-nucleolytic activity in the SNM1 nuclease family Article Snippet: .. 10 pmol of single-stranded DNA (Eurofins MWG Operon, Germany) were labelled with 3.3 pmol of α- 32 P-dATP (Perkin Elmer) by terminal deoxynucleotidyl transferase (TdT, 20 U; ThermoFisher Scientific), incubated together at 37°C for 1 h. This solution was passed through a Article Title: SNM1A is crucial for efficient repair of complex DNA breaks in human cells. Article Snippet: .. Substrates were prepared as follows: 10 pmol of DNA oligonucleotide (Eurofins MWG Operon, Germany) were 3’-end labelled with 3.3 pmol of α-32P-dATP (Perkin Elmer) using terminal deoxynucleotidyl transferase (TdT, 20U; ThermoFisher Scientific), incubated together at 37 °C for 1 h. Unincorporated nucleotides were removed using a Article Title: Characterization of the SARS-CoV-2 ExoN (nsp14 ExoN –nsp10) complex: implications for its role in viral genome stability and inhibitor identification Article Snippet: .. 10 pmol of single-stranded (ss) RNA or DNA (Eurofins MWG Operon, Germany) was incubated with 6.8 pmol γ- 32 P-ATP (Perkin Elmer), and 10 U T4 PNK (ThermoFisher Scientific) at 37°C for 1 h. This solution was passed through a Article Title: Cell-active small molecule inhibitors validate the SNM1A DNA repair nuclease as a cancer target Article Snippet: .. 3′-Radiolabelled substrates were made by labelling 10 pmol of single-stranded DNA (Eurofins MWG Operon, Germany) with 3.3 pmol of α- 32 P-dATP (PerkinElmer) by incubation with terminal deoxynucleotidyl transferase (TdT, 20 U; ThermoFisher Scientific), at 37 °C for 1 hour, before being passed through a Article Title: Molecular insights into the stimulation of SNM1A nuclease activity by CSB during interstrand crosslink processing Article Snippet: All substrates, except the ‘0-nucleotide’ (herein referred to as ‘0-nucleotide’) ICL-containing substrate (Fig. S1 and Table S1), were 3′-labelled using terminal deoxynucleotide transferase (TdT) (NEB), then annealed with the complementary oligonucleotide. .. For labelling, oligonucleotide (10 pmol), TdT (20 U) and α- 32 P-dATP (3.3 pmol) were prepared in 1 x TdT buffer (NEB) and incubated at 37 °C for 1 h. The reaction mixture was then passed through a Article Title: SNM1A is crucial for efficient repair of complex DNA breaks in human cells Article Snippet: .. Substrates were prepared as follows: 10 pmol of DNA oligonucleotide (Eurofins MWG Operon, Germany) were 3’-end labelled with 3.3 pmol of α- 32 P-dATP (Perkin Elmer) using terminal deoxynucleotidyl transferase (TdT, 20 U; ThermoFisher Scientific), incubated together at 37 °C for 1 h. Unincorporated nucleotides were removed using a Chromatography:Article Title: SNM1A ( DCLRE1A ) is required for the efficient repair of complex DNA breaks in human cells Article Snippet: .. Substrates were prepared as follows: 10 pmol of DNA oligonucleotide (Eurofins MWG Operon, Germany) were 3’-end labelled with 3.3 pmol of α-32P-dATP (Perkin Elmer) using terminal deoxynucleotidyl transferase (TdT, 20 U; ThermoFisher Scientific), incubated together at 37°C for 1 h. To remove unincorporated nucleotides, these were passed through a Article Title: A phosphate binding pocket is a key determinant of exo- versus endo-nucleolytic activity in the SNM1 nuclease family Article Snippet: .. 10 pmol of single-stranded DNA (Eurofins MWG Operon, Germany) were labelled with 3.3 pmol of α- 32 P-dATP (Perkin Elmer) by terminal deoxynucleotidyl transferase (TdT, 20 U; ThermoFisher Scientific), incubated together at 37°C for 1 h. This solution was passed through a Article Title: SNM1A is crucial for efficient repair of complex DNA breaks in human cells. Article Snippet: .. Substrates were prepared as follows: 10 pmol of DNA oligonucleotide (Eurofins MWG Operon, Germany) were 3’-end labelled with 3.3 pmol of α-32P-dATP (Perkin Elmer) using terminal deoxynucleotidyl transferase (TdT, 20U; ThermoFisher Scientific), incubated together at 37 °C for 1 h. Unincorporated nucleotides were removed using a Article Title: Characterization of the SARS-CoV-2 ExoN (nsp14 ExoN –nsp10) complex: implications for its role in viral genome stability and inhibitor identification Article Snippet: .. 10 pmol of single-stranded (ss) RNA or DNA (Eurofins MWG Operon, Germany) was incubated with 6.8 pmol γ- 32 P-ATP (Perkin Elmer), and 10 U T4 PNK (ThermoFisher Scientific) at 37°C for 1 h. This solution was passed through a Article Title: Cell-active small molecule inhibitors validate the SNM1A DNA repair nuclease as a cancer target Article Snippet: .. 3′-Radiolabelled substrates were made by labelling 10 pmol of single-stranded DNA (Eurofins MWG Operon, Germany) with 3.3 pmol of α- 32 P-dATP (PerkinElmer) by incubation with terminal deoxynucleotidyl transferase (TdT, 20 U; ThermoFisher Scientific), at 37 °C for 1 hour, before being passed through a Article Title: Molecular insights into the stimulation of SNM1A nuclease activity by CSB during interstrand crosslink processing Article Snippet: All substrates, except the ‘0-nucleotide’ (herein referred to as ‘0-nucleotide’) ICL-containing substrate (Fig. S1 and Table S1), were 3′-labelled using terminal deoxynucleotide transferase (TdT) (NEB), then annealed with the complementary oligonucleotide. .. For labelling, oligonucleotide (10 pmol), TdT (20 U) and α- 32 P-dATP (3.3 pmol) were prepared in 1 x TdT buffer (NEB) and incubated at 37 °C for 1 h. The reaction mixture was then passed through a Article Title: SNM1A is crucial for efficient repair of complex DNA breaks in human cells Article Snippet: .. Substrates were prepared as follows: 10 pmol of DNA oligonucleotide (Eurofins MWG Operon, Germany) were 3’-end labelled with 3.3 pmol of α- 32 P-dATP (Perkin Elmer) using terminal deoxynucleotidyl transferase (TdT, 20 U; ThermoFisher Scientific), incubated together at 37 °C for 1 h. Unincorporated nucleotides were removed using a Article Title: Molecular insights into the stimulation of SNM1A nuclease activity by CSB during interstrand crosslink processing Article Snippet: The oligonucleotide (10 pmol) was incubated with Klenow (exo-) (12.5 U, NEB) and α- 32 P-dATP (3.3 pmol) in 1 x NEB buffer 2 at 37 °C for 45 min. dGTP (1 mM) and dATP (1 mM) were added to 0.1 mM final concentrations and the mixture incubated for 30 min at 37 °C. .. The reaction was then passed through a Concentration Assay:Article Title: Molecular insights into the stimulation of SNM1A nuclease activity by CSB during interstrand crosslink processing Article Snippet: The oligonucleotide (10 pmol) was incubated with Klenow (exo-) (12.5 U, NEB) and α- 32 P-dATP (3.3 pmol) in 1 x NEB buffer 2 at 37 °C for 45 min. dGTP (1 mM) and dATP (1 mM) were added to 0.1 mM final concentrations and the mixture incubated for 30 min at 37 °C. .. The reaction was then passed through a |